Specific N-terminal ACTH-radioimmunoassay after plasma extraction
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چکیده
منابع مشابه
C- and N-terminal specific LH-releasing hormone (LRH) radioimmunoassay.
A highly sensitive and specific LRH radioimmunoassay is described. Three antisera were compared and characterized. High specificity was shown by 2 of them in that both C- and N-terminals were required for binding when testing LRH analogs. A lower detection limit of about 1 pg could be obtained using a simple organic solvent precipitation method for separation of bound/free antigen. Good correla...
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A radioimmunoassay specific for the amino (N) terminal portion of the parathyroid hormone (PTH) molecule (N-PTH radioimmunoassay) has been developed by iodinating synthetic 1-34bovine PTH (1-34bPTH) and using commercially available bPTH antiserum. A radioimmunoassay specific for the carboxyl (C) terminal (C-PTH radioimmunoassay) has been carried out by adding enough amount of 1-34bPTH to the PT...
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Interference by human plasma proteins in the radioimmunological determination of somatostatin was eliminated by subjecting plasma samples to acid--ethanol precipitation. The assay was performed on the lyophilized supernate from 300 microliters of human plasma, with reagents that are commercially available. Sensitivity was 2.6 pg, corresponding to 8.7 ng/L of plasma. The intra-assay CV was 8%; t...
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ژورنال
عنوان ژورنال: Fresenius' Zeitschrift f�r Analytische Chemie
سال: 1980
ISSN: 0016-1152,1618-2650
DOI: 10.1007/bf00467772